MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plate

• 3-Methoxytyramine
• Metanephrine
• Normetanephrine

6PLUS1^® Multilevel Calibrator Set and MassCheck^® controls based on human plasma

Isotopically labelled internal standard for each analyte

Fully automated workflow solution available
 

DESCRIPTION

MassChrom^® Free Metanephrines in Plasma
With 96 SPE Well Plates, suitable for manual and automated sample preparation
For 2 x 96 determinations

Clinical relevance

This kit allows the quantitative determination of metanephrine, normetanephrine and 3-methoxytyramine in plasma by LC-MS/MS.

One typical application for this kit is the analysis of patient samples with a suspicion of pheochromocytomas. These tumours and other neural crest tumours (e.g. paragangliomas and neuroblastomas) secrete metanephrine, normetanephrine and 3-methoxytyramine. The plasma free metanephrines are more stable and secreted more evenly than catecholamines (epinephrine, norepinephrine, and dopamine). Some studies show that the analysis of plasma free metanephrines has the highest sensitivity and specificity in pheochromocytoma diagnostics compared to plasma free catecholamines and metanephrines in urine.

Product advantages

• 6PLUS1® Multilevel Calibrator Set and MassCheck^® controls based on human plasma
• Simple sample prep in just a few steps
• Chromatographic separation of analytes – no co-elution
• Isotopically labelled internal standards for all analytes
• Fully automated workflow solution available

This kit encompasses all required components and allows a simple and fast sample preparation: solid phase extraction (SPE) without equilibration/conditioning steps, analyte enrichment without evaporation step, only one elution step. The analytes are chromatographically separated to avoid co-elution and to minimise risks of interferences. The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantification. The 6PLUS1^® Multilevel Plasma Calibrator Set and MassCheck^® controls ensure high precision of results. They are obtained from human plasma to minimise matrix-induced differences.

A fully automated workflow solution is also available.

Alternatively, there is also a method available using Sample Clean Up Columns (81000/C).

CHROMATOGRAM

TECHNICAL DATA

Method of Analysis

Number of Tests

Parameter

Analysis Time

Limit of quantification

Metanephrine: 15 ng/l
Normetanephrine: 7 ng/l 
3-Methoxytyramine: 11 ng/l

Linearity

Metanephrine: up to 5650 ng/l
Normetanephrine: up to 8500 ng/l 
3-Methoxytyramine: up to 5800 ng/l

Recovery

Intraassay

Interassay

Gradient

Specimen

Sample Preparation

• Pipette 500 µl Dilution Buffer and 25 µl Internal Standard Mix into each well of the 96 SPE Well Plate.
• Add 500 µl of the sample/calibrator/MassCheck^® control, centrifuge, discard effluent.
• Add 0.9 ml Wash Buffer 1, centrifuge, discard effluent.
• Add 2 x 0.9 ml Wash Buffer 2, centrifuge, discard effluent.
• Add 250 µl Elution Buffer into each well and centrifuge.
• Inject up to 25 µl.

Injection Volume

Ionisation

MS/MS-Mode

Please note

KIT COMPONENTS

Components available separately

Mobile Phase A

Order no.: 81001

DETAILS

Mobile Phase B

Order no.: 81002

DETAILS

Dilution Buffer

Order no.: 81003

DETAILS

Internal Standard Mix

Order no.: 81004

DETAILS

Wash Buffer 1

Order no.: 81005

DETAILS

Wash Buffer 2

Order no.: 81006

DETAILS

Elution Buffer

Order no.: 81007

DETAILS

Rinsing Solution

Order no.: 81009

DETAILS

96 SPE Well Plate

Order no.: 81057

DETAILS

Collection Plates

Order no.: 81058

DETAILS

Pierceable adhesive seals for 96 Well Plates

Order no.: 81059

DETAILS

ACCESSORIES

Analytical Column

Order no.: 81100

DETAILS

Tuning Mix Free Metanephrines in Plasma

Order no.: 81015

DETAILS

System Check Solution

Order no.: 81010

DETAILS

Waste Plates

Order no.: 81056

DETAILS

Stainless steel prefilter housing

Order no.: 15070

DETAILS

Stainless steel prefilter - 0.5 µm

Order no.: 15071

DETAILS

CALIBRATORS

Calibrators available separately

6PLUS1® Multilevel Plasma Calibrator Set Free Metanephrines

Order no.: 81039

DETAILS

CONTROLS

Controls available separately

MassCheck® Free Metanephrines Plasma Controls

DETAILS